Plasmid
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Description
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Map |
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pBSII-ITR1.1k |
The new piggyBac minimal construct with only 235bp 3’ TR
and 310bp 5’ TR. |
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pBSII-ITR1.1k-ECFP |
The pBSII-ITR1.1k minimal construct with 3xP3-ECFP genetic
marker. Tested in Drosophila melanogaster with high transformation
frequency (up to 36%). |
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pBSII-ITR1.1k-EYFP |
The pBSII-ITR1.1k minimal construct with 3xP3-EYFP genetic
marker. |
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pXL-Bac II |
The new piggyBac minimal construct in a more traditional
format (based on pBSII-ITR1.1k) |
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pXL-BacII-ECFP |
The pXL-Bac II minimal construct with 3xP3-ECFP genetic marker.
Tested in Drosophila melanogaster with high transformation frequency
(up to 25%). |
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p3E1.2 |
The original baculovirus fragment clone with the intact piggyBac
element and flanking viral sequence. |
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pCasper-hs-orf |
The hsp70 promoter controlled piggyBac open reading
frame in a Drosophila P-element construct. Tested and works very well as
helper. |
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pBSII-hs-orf |
Alternative hsp70 promoter driven piggyBac ORF
helper plasmid. |
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pBSII-IFP2-orf |
The piggyBac ORF under control of the T7 promoter in the pBlueScript cloning vector. |
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pBSII-IE1-orf |
The IE1 baculovirus promoter driven piggyBac ORF helper
plasmid. |
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pBSII-Act5c-orf |
The Drosophila Actin 5c promoter driven piggyBac ORF
helper plasmid. |
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p3xP3-DsRed1-orf |
The CMV promoter driven piggyBac ORF helper plasmid. Also
contains a 3xP3 controlled DsRed1 genetic maker. |
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